Expression of senescence-associated genes in treated broccoli microgreens after 0, 4, 7, 14 d of storage at 5 °C. Total RNAs used for reverse transcription were isolated from microgreens on different days post-harvest. Transcript levels of four senescence-associated genes were determined by RT-qPCR. Relative gene expression levels (highest value = 100%) are shown following normalization with house-keeping gene actin transcript values. For each gene, different letters indicate statistically significant differences among mean values (P value < 0.05; t-test). The RT-qPCR analyses were repeated at least three times from two independent experiments with similar results