for Microbacterium oxydans HG3, Ochrobactrum sp. strain HG16, Lysinibacillus sp. strain HG17, Bacillus sp. strain CM111, and Serratia marcescens
HG19 and with 10 M HgCl2 for Kocuria rosea EP1 and Bacillus cereus MM8. After incubation for 24 h at 30°C, colonies were harvested and were resuspended in 100 l 0.07 M phosphate buffer (pH 7), containing 0.5 mMEDTA, 0.2mMmagnesium acetate, and 5mMsodium thioglycolate, either without HgCl2 or with HgCl2 at concentrations up to 100 M.
The suspensions were diluted with buffer to give an optical density of 0.2 at 620 nm.