FAMEs were analysed on a Varian gas chromatography 3900 equipped with flame ionization detector. Fatty acids methyl esters are polar compounds in nature; therefore, a highly polar column,
DB-FFAP from J andWScientific, Agilent Technologies (Santa Clara,USA) was selected for the analysis. This column consists of a polyethylene glycol bonded nitroterephthalic acid phase. The
dimensions of the column were 30 m 0.53 mm i.d., 0.50 lm film thickness. Helium was used as the carrier gas at a flow rate of 1.2 mL/min. The temperature program was as follows: initial tem-
perature 60 C for 3 min, increased by 5 C/min to final temperature 240 C and held for a further 5 min. The detector temperature was 260 C. The injector was set at 250 C with split
ratio of 20:1. Star Chromatography Workstation Version 6.3 (Varian, USA) was used for data collection, and calculation of all parameters. 26 FAMEs were analysed from SRM 2377 mixture with a total run time of 44 min.