Bacillus thuringiensis (Bt), a gram-positive, spore-forming
bacterium, produces a variety of insecticidal crystal
proteins (ICP) toxic to lepidopteran insects (Kumar et al.,
1996). The ICP genes of Bt have been successfully engineered
into many crop plants to yield resistance against
lepidopteran insects (Kumar and Sharma, 1994). The
levels of toxin expression in plants, however, have been
insu$cient when the native genes were used, necessitating
the use of a truncated version of the genes and
modi"cation of the coding sequence, such as removal of
potential RNA processing and polyadenylation signals
and optimization of the codon usage to achieve higher
expression of the gene in plants (Perlak et al., 1991).
When expressed in plant cells, these modi"ed genes conferred
signi"cant protection against insects to important
crops such as cotton, maize, rice, potato and eggplant
Bacillus thuringiensis (Bt), a gram-positive, spore-formingbacterium, produces a variety of insecticidal crystalproteins (ICP) toxic to lepidopteran insects (Kumar et al.,1996). The ICP genes of Bt have been successfully engineeredinto many crop plants to yield resistance againstlepidopteran insects (Kumar and Sharma, 1994). Thelevels of toxin expression in plants, however, have beeninsu$cient when the native genes were used, necessitatingthe use of a truncated version of the genes andmodi"cation of the coding sequence, such as removal ofpotential RNA processing and polyadenylation signalsand optimization of the codon usage to achieve higherexpression of the gene in plants (Perlak et al., 1991).When expressed in plant cells, these modi"ed genes conferredsigni"cant protection against insects to importantcrops such as cotton, maize, rice, potato and eggplant
การแปล กรุณารอสักครู่..
