REAGENT PREPARATION
1. To prepare substrate solution, make an 1:1 mixing of Reagent A
with Reagent B right before use. Mix gently to ensure complete
mixing. Discard excess after use.
2. Dilute 1 volume of Wash Buffer (50x) with 49 volumes of distilled
water. For example, Dilute 15 ml of Wash Buffer (50x) into 735 ml
of distilled water to prepare 750 ml of washing buffer (1x). Mix well
before use