Different parts of mother plants can be chosen to establish an asparagus clone
through in vitro culture, e.g. anthers [Doré 1977; Tsay et al. 1980; Inagaki et al. 1981;
Reuther 1983], shoot segments [Harada and Yakuwa 1983], bud clusters [Kohmura et
al. 1994]. Callus induction efficiency varies in explant materials.