1
HPLC analysis of Xcn1
on a reversed-phase C
18
column
(Waters 2695 system). Samples
of 5
l
L were subjected to a
column (2.1
9
150 mm), eluted
with acetonitrile-0.1% TFA
(V:V
=
10:90), and monitored
at 312 nm. One main peak
appeared at a retention time of
9 min. The calculated purity is
greater than 90% according to
the peak area