Earthworms were washed in fresh water and sorted on the presence or absence of clitellum. Clitellated earthworms were narcotized in 70% ethyl alcohol and fixed in 5% formalin for 6–8 h and finally preserved in 5% formalin. The preserved samples were studied morphologically and dissected for study diagnostic taxonomic character such as spermathecae (number and location), prostate gland (location and shape), prostomium shape, and clitellum position.