2.9. Distribution of carbon derived from 14C-glucose or 14C-mannitol
Cut spikes were held in 0.25 M glucose or mannitol solution for ten days as described above. The cut ends of spikes were transferred into 14C-glucose or 14C-mannitol solution (23.135 kBq, 185 MBq mmol1) for 1 h. The spikes were then transferred in to 250 mM glucose or 250 mM mannitol solution and kept at 23 C for 24 h in darkness. The spikes were divided into five portions: basal stem from cut end to 30 cm, flowers generated on the basal stem, remaining upper stem, flowers on remaining upper stem, and tips with length of 1 cm. These portions were dried at 80 C for more than 3 days. The dried samples were ground and 20 mg subsample was oxidized and dissolved in a liquid scintillator with a sample combustion system (ASC-113, Aloka). The radioactivity of the solution was measured with a liquid scintillation counter (LSC-3500, Aloka).
2.9. Distribution of carbon derived from 14C-glucose or 14C-mannitolCut spikes were held in 0.25 M glucose or mannitol solution for ten days as described above. The cut ends of spikes were transferred into 14C-glucose or 14C-mannitol solution (23.135 kBq, 185 MBq mmol1) for 1 h. The spikes were then transferred in to 250 mM glucose or 250 mM mannitol solution and kept at 23 C for 24 h in darkness. The spikes were divided into five portions: basal stem from cut end to 30 cm, flowers generated on the basal stem, remaining upper stem, flowers on remaining upper stem, and tips with length of 1 cm. These portions were dried at 80 C for more than 3 days. The dried samples were ground and 20 mg subsample was oxidized and dissolved in a liquid scintillator with a sample combustion system (ASC-113, Aloka). The radioactivity of the solution was measured with a liquid scintillation counter (LSC-3500, Aloka).
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