The test was performed according to method described previously (16 – 17) with a minor modification. Fifty microlitres of formaldehyde 2.5% (v/v in distilled water) was injected subcutaneously into a hind paw of mice, 30 min after administration of the extract (250 and 500 mg/kg, p.o.) and diclofenac sodium (25 mg/kg, p.o.) or vehicle. The time (in seconds) spent in licking, biting and scratching responses of injected paw was considered as an indicator of pain response. Responses of the first 5 min was considered as early phase (neurogenic phase) and the period of 15–30 min as the late phase (inflammatory phase). The following formula was used for the calculation of percent inhibition,