Antioxidant activity of optimized GAS protein hydrolysate
Antioxidants play an important role in food industries for purposes of nutritional preservation and prevention of color and flavor deterioration. Analysis of antioxidant activity on GAS protein hydrolysate showed higher ferrous chelating (46.80%) and hydroxyl radical scavenging activities (80.58%) compared to BHT (36.09% and 51.93%), respectively (data not shown). However, BHT showed higher reducing power (2.52%) compared to GAS protein hydrolysate (0.62%). The ferrous ion (Fe2+) is a pro-oxidant that interacts with hydrogen peroxide (Fenton reaction) to produce reactive oxygen species (ROS) and the hydroxyl (OH) free radical, which may initiate and/or accelerate lipid oxidation (Stohs, 1995). The complex formation of the ferrous ion is