2.3.3. Agar well diffusion method
Antifungal screening was carried out using the agar well
diffusion assay. Twenty ml of sterilized sabouraud dextrose
agar medium poured into a 15 cm Petri dish. Twenty L
of inoculum suspension of the T. rubrum was distributed
evenly over the surface. A 6mm well was cut in the centre
of each plate using a sterilized cork borer. 50 L of plant
extracts and Griseofulvin (Dr.Reddy’s Labs, India) were
placed into the wells. The plates were incubated for 5
days at 28 ํc. Pure organic solvents were used as control.
Results were determined based on size of the inhibitory
zone surrounding the wells containing the test solution. The
diameter of zones of inhibiti