Two volumes of 96% w/w ethanol were added
to the filtrate for pectin precipitation and the obtained mixture
was kept for 1 h at 4 C. After centrifugation at 8000 g for
20 min at 10 C, the pectin precipitate was washed with 50%, 75%
and two times with 100% ethanol and centrifuged at 5000g for
10 min at 10 C. Finally, the obtained pectin was dried at 45 C to
a constant weight, ground in a mortar and subjected to further
analyses.