2.5. Screening for transformed plants using PCR
DNeasy plant mini kit (Qiagen) was used to isolate DNA from
newly emerged cotton leaves. The template DNA was used for
PCR amplification with bC1gene (antisense) primer F-T
CACCATCGCTAATCAAGTATG and R-CATTGCTGGT
TTGTGTTTGGAA. The PCR reaction was performed in a
mixture, containing 1.0 ll DNA (50 ng) 2.0 ll buffer (10·),
2.0 ll dNTPs (10 Mm), 0.5 ll of 100 ng forward and reverse
primer, 0.5 ll of 2.5U Taq DNA polymerase. The following