Figure 4. (A) Uptake of AuNP-E by RAW264.7 cells determined from ICP-AES (mean ± SEM, n = 3). TEM images of RAW264.7 cells treated with 2 mg/mL AuNP-Esfor 3 h; (B) Sphere20-E, (C) Sphere40-E, (D) Cube-E, and (E) Rod-E. Arrows indicate AuNP-E in the cytosol. Scale bar represents 300 nm. (F) Exocytotic level of AuNP-Es 48 h after exposure to AuNP-E for 1.5 h. Fexo = Nout/N0, where Nout is the number of NPs exocytosed from the cells and N0 is the number of NPs internalized before exocytosis (means ± SEM, n = 3).(22)