Hemoglobin Concentration (Hb) was
determined using Hemoglobin –Drabkin Kit. The
Packed Cell Volume% (PCV) of Erythrocytes of
whole blood was measured using a
microhaematocrict centrifuge (Hawksley,London).
The Erythrocytes (RBC) were counted using
Hayems solution. Mean Corpuscular Volume
(MCV), Mean Corpusular Heamoglobib (MCH) and
Mean Corpuscular Haemoglobin Concentration
(MCHC)% also were calculated. Plasma glucose
and cholesterol were determine by enzymatic
calorimetric methods using Kit GOD-PAP (Randox
Labratoty Ltd. London). Plasma total protein was
determined as shown by (King and Wootton, 1965).
Plasma triglyceride was determined by the methods
described by Buccolo et al., (1973). Creatinine was
determined using commercial kits (Biosystem
Reagents and Instruments). Calcium was
determined by colorimetric method described by
Gindler and King (1972). Phosphorous was
determined by the method described by (Gamst and
Try, 1980), (Farrell and Kaplan et al., 1984).