The recN gene has been shown to have a lower degree of similarity at the species level and higher divergence value at the subspecies level than other housekeeping genes ( Glazunova et al., 2010). In accordance with these findings, Le et al. (2013) reported that recN sequence analysis revealed complete concordance with the DNA-DNA reassociation results. These results suggested that the recN gene could be a suitable target for the rapid detection of S. suis.
We here describe a novel PCR method that targets the recN gene of S. suis, designated recN PCR, and evaluated its potential as an identification system based on a current taxonomical study.