Material and methods
Incubation of A. oryzae
Th e A. oryzae used in this study was kept in our
laboratory, isolated from a cow rumen, and identifi ed
by 26 S rDNA. Th e A. oryzae was incubated at 30 °C
for 3 days in potato dextrose agar (PDA) medium
(0.6% soluble starch, 0.2% yeast extract, 0.5%
peptone, 2% dextrose, 2% agar, 0.2% KH2
PO4
, 0.03%
MgSO4
·7H2
O). Spore suspension was obtained
by scraping off the spores from pure cultures and
suspending them in sterile 0.9% physiological saline.
Spore counts were determined to be approximately 1
× 109
cfu/mL