Total RNA was extracted from different tissues with a Simply P
total RNA extraction kit (BioFlux, China) according to the manufacturer’s
procedure. Three replicates of RNA extraction were conducted.
Genomic DNA was eliminated from total RNA with
RNase-free DNase I, and first-strand cDNA was synthesized with
the M-MLV (RNase H) (TaKaRa, Japan) and oligo-dT primers.