Loop-mediated isothermal amplification (LAMP) is an isothermal amplification technique that can be undertaken with minimal
equipment to obtain amplification of target DNA within 30 minutes. A range of assays for specific 16Sr phytoplasma groups,
which when combined with rapid DNA extraction techniques can result in detection of the phytoplasma in plant material within 1
hour of sampling was developed. A range of alternative methods are available for detection of the amplification product including
incorporation of hydroxyl napthol blue into the reagent mix, agarose gel electrophoresis and real-time detection systems. The advantage
of the real-time method is that tubes do not have to be opened, minimising the risk of contamination of samples.