Next, we examined the simultaneous enzymatic hydrolysis and
fermentation of soybean molasses (SHF of SM). Preliminary experiments
were performed to determine the optimal concentration of
SM, the kinetic profiles of fermentation and a CCD to assess the
influence of the enzyme concentration, amount of inoculum and
temperature in Y1 and RS. These experiments were also conducted
in a batch reactor with the initial pH adjusted to 4.5 and the concentration
of soybean molasses determined by the test concentration
of hydrolyzed soybean molasses. The amount of enzyme used (X1)
ranged from 0.009 to 0.091% based on the weight of the substrate,
the inoculum concentration (X2) ranged from 14.7 to 55.3 g/L, and
the temperature (X3) ranged from 25.5 to 44.5 ◦C. The alpha orthogonality
used in CCD was 1.353.
The actual factor levels corresponding to coded factor levels are
shown in Table 2.