After removing the germ and grain ends, the brown rice was
soaked in water for 12 h to reduce the adhesion between different
layers. Then the grain was cut into two pieces by a razor-blade, and
the pericarp and aleurone layer were peeled off gently by using a
tweezer and a scalpel. Starchy endospermwas scraped off from the
peeled part by a scalpel. The isolated tissues were defatted using
acetone with a material to solvent ratio 1:8. After drying in an oven
at 50 C, the tissues were analysed for arabinoxylans content