Results and discussion
Chemical analyses
Chemical analyses of nitrates and pesticides were performed as part of regular drinking water monitoring by The national institute of health of Republic of Slovenia, Department of sanitary chemistry – the accredited institution for chemical analyses of drinking water in Slovenia. The concentrations of all measured parametersinTable1werebellowthemaximumallowed concentration (MAC) of chosen chemical analytical methods for all water samples according to Slovenian regulations 17,18,19
Analyses (Table 1) were performed by The institute of public health of the Republic of Slovenia, Department of sanitary chemistry. Methods of the chemical analyses (Table 1) are part of the Annex to the Accreditation Certificate No. L -052 (LP-029). Nitrates were analyzed by molecular absorption spectrometric standard method (SM-NO3-4500B)31 pesticides and their metabolites were analyzed by gas chromatography with mass spectrometry detection (SIST EN ISO 10695).32
The results of the three biotests
Short-term mutagenicity test are widely applied in the analyses of complex environmental mixtures as sensitive tools for the detection of trace amounts of contaminants or unknown components with genotoxic