2.6. Preparation of pork patties and experimental design
After thawing at 4 °C, LD muscle was cut in small cubes and minced
twice through a 4 mm plate (Stiebel Eltron International, KFW3i/03121,
Germany). Using minced meat from treatment (L), 5 groups of pork
patties were prepared, namely a control group (CONT), a group with
added α-tocopherol at 50 mg/kg (ATOC), and 3 groups with added
olive leaf extracts containing total polyphenols at 100 mg GAE/kg
(group TP100), 200 mg GAE/kg (group TP200) and 300 mg GAE/kg
(group TP300), respectively. In the CONT group, the ingredients per
kilogram of patties were 965 g of meat, 17 ml of distilled water, 3 ml
of ethanol and 15 g of sodium chloride. In the formulation of the treated
patties, the volumes of pure ethanol had been replaced by equal volumes
of ethanol containing the corresponding antioxidants.