Deamidated wheat gliadin was prepared according to Qiu, Sun,
Zhao, et al. (2013). Citric acid (0.2 M) was added to wheat gluten
solution (8 wt%) with constant stirring in a sealed glass container
at 70 C for 16 h. The dispersion was then neutralized, dialyzed,
and freeze-dried. The gliadin-rich fraction was extracted from
deamidated wheat gluten by stirring with 70% ethanol for 2 h.
The ethanol in the supernatant was removed by rotary evaporation
(50 C). The final deamidated gliadin was obtained by freeze-drying.