and solvent system was mixed properly by gentle stirring on magnetic
stirrer for 30 min. In order to form three phases, system was
allowed to stand at 45 C in a water bath for 1 h. The three phases
formed were separated by centrifugation at 2900g for 10 min at
30 C. The upper organic layer of t-butanol containing extracted
fat was collected and the t-butanol was evaporated on a rotary
evaporator to obtain the extracted fat. The amounts of fat recovered
by Soxhlet extraction was considered as 100% while calculating
amount of fat recovered by TPP. One factor at- a- time method
was used to study effect of different parameters and is detailed in
Table 1. All extractions were carried out in triplicates.