The methanolic extracts of Holoptelea integrifolia (Roxb.) (Urticaceae) leaves (MLE) and stem bark (MSBE) were studied for the woundhealing
potential. Since wound healing is severely hampered by microbial infection and reactive oxygen species (ROS), this study was undertaken
to evaluate antimicrobial and antioxidant activity apart from wound-healing activity. The antimicrobial property of the Holoptelea was studied
against the six bacterial and five fungal strains using the agar well diffusion method and minimum microbicidal concentration and minimum
inhibitory concentration were determined for each strain, in which methanolic extract of stem bark (MSBE) has shown bigger zone of inhibition
(11.3–20.4 mm) than methanolic extract of leaves (MLE) (9.6–14.9 mm). The anti-oxidant activity was evaluated by DPPH free radical scavenging
activity using HPLC method. The IC50 values obtained for MSBE (TPC: 78.53±1.26 mg/g) and MLE (TPC: 57.71±1.45 mg/g) were 37.66±0.48
and 50.36±0.59g/well, respectively. In excision wound model, more than 90% wound healing was recorded in treated groups by 14 days of
post surgery, where as only 62.99% was observed in the control group. In incision model, higher breaking strengths and higher hydroxyproline
content in treated groups suggested higher collagen re-deposition than the control group. Finally, histopathology studies conformed wound-healing
activity of Holoptelea integrifolia.