mixtures were incubated at 37 1C in a shaking water bath for
3 h. After incubation, 10mL of diethyl ether were added to
stop reactions and to extract the reaction products. The
ether extracts were concentrated by evaporation and applied
to a silica-gel 60 thin-layer chromatography (TLC) plate
(Merck). Pure tri-, di- and monopalmitin (Sigma) were used
as reference glycerides. The TLC plates were developed with
chloroform/acetone/acetic acid (96 : 4 : 1, by volume). The
spots of acylglycerols and fatty acids were visualized by
exposure to iodine vapor.