All SCCO2 extractions were carried out in dynamic mode with a
constant decompressed CO2 flow rate of 400 mL/min (residence
time between 4.9 and 14.1 min depending on fluid density).
The pressure was varied from 10 to 50 MPa between experiments
and the temperature was set to either 60 or 80 C. Extractions
lasted between 80 and 120 min. For each extraction, a new pre weighed
glass vial for lipid collection was used every 20 min.
The crude lipid in each vial was gravimetrically quantified and analysed
for FAME profiles.