The organizational culture change process—through a learning organization promoting knowledge transfer in the work envi- ronment and collective learning—instituted in a CSSS of Quebec brought about important and much needed changes in nursing practices and work environment. Research on new and innovative solutions to improve knowledge management and support con- tinuing professional development of health care professionals is still limited. This case study can help clarify the impacts of a learn- ing organization on health care professionals’ practices. According to the participants in this case study, the learning organization seemed to affect daily nursing work in a positive manner, despite its variable impact on other professionals and other sites outside the hospital center. These changes were particularly pronounced with respect to knowledge transfer, support for nursing practices, and quality of health care objectives, which the learning organi- zation had sought to meet since its inception. As a final remark, it would be interesting to study in the future the longer-term effects of the integration of a learning organization on nurses’ professional development, as well as on those of other health care professionals who are part of this new culture.