the extract dialyzed against dH2O for 6 to 8 h with hourly changes of dH2O, and 17.5 g of (NH4)2SO4 100 ml−1of dialyzate was added to precipitate the protein. After 2 to 3 h, the precipitate was centrifuged at 27,000 g, resuspended in a minimum amount of dH2O, dialyzed for 8 h against dH2O with several changes of dH2O, and lyophilized.