Cell immobilization on BSG and DBSG was carried
out by suspending 16 g of S. cerevisiae AXAZ-1 cells in
800ml of glucose synthetic medium, containing 4 g/l yeast
extract, 1 g/l (NH4)2SO4, 1 g/l KH2PO4, 7 g/l MgSO4 ·7H2O
and 120 g/l glucose monohydrate, and mixing with 200 g of sterilized deligniWed BSG (DBSG) or non-deligniWed BSG
(BSG2).