The H-point curve isolation method (HPCIM) for the
detection of unknown interferences in chromatography is
proposed. The method allows one to estimate the UV-
vis spectra of interfering species in a sample as well as to
test the purity of the chromatographic peaks. Besides the
detection of the unknown interferences in a sample, this
method allows one to calculate the concentration of an
analyte in the presence of unknown compounds. To
illustrate the reliability of the proposed method, samples
of diuretics and amphetamines have been analyzed by
normal- and reversed-phase high-performance chroma-
tography.