Industrial wastewater samples were collected in sterilized
screw capped bottles from different industrial areas of Sheikhupura
and Kotlakhpat, Lahore, Pakistan. Some physiochemical parameters
of wastewater samples viz., temperature (C), pH and cadmium ion
(Cdþ2
) concentration (mM) were monitored. Cdþ2 resistant yeast
was isolated and maintained in Yeast Potato Dextrose (YPD) agar
(1% yeast extract, 2% peptone, 2% glucose and 2% agar) supplemented
with 1 mM Cdþ2 at 30 C. The 48 h grown culture in YPD
agar was used as the inoculum for further experiments.