2.5. Scanning electron microscopy
Scanning electron microscopy was carried out following the
method described by Karnovsky[21] under high vacuum using a
microscope Zeiss Supra 55VP (Carl Zeiss, Oberkochen, Germany).
Samples were processed and observed in the Centro Integral de
Microscopía Electr onica (CIME), CCT-CONICET-UNT, San Miguel de
Tucum an (Argentina).
2.6. Statistical analysis