All tubes with media
were autoclaved at 121°C and 1.04 kg/cm²
for 15 minutes. Apical meristems were
divided longitudinally into four equal
segments aseptically inside laminar air flow
cabinet and cultured on callus initiation
medium for 24 weeks and recultured at 4
weeks intervals (Fig. 1-A and B). Initial calli
were then inoculated into 250 ml
polycarbonate jars containing 40 mL of
embryogenic callus proliferation medium
(Fig. 1-C).