Overall, the development of a probiotic product comprising microorganisms selected for their CE potential seems a promising approach to fulfill both the objectives for food safety and enhanced broiler performance. In this sense, a new multi species probiotic product has been developed under a European-funded research project. To account for gut dynamics,the product is formulated using 5probiotic species isolated from the crop,jejunum,ileum, and cecum of healthy adult fowl.Species selection criteria includes satisfactory growth and stability, performance in cocultivation with a range of common pathogens,antibiotic resistances,and virulence factors(Kloseetal.,2006). The aim of this work was to evaluate the efficacy of the multi bacterial species probiotic product in broiler nutrition. Broiler performance parameters such as BW, feed intake (FI), and feed conversion ratio (FCR) were determined. In addition, because chicken ceca are the most heavily populated GI tract region (Mead, 2000), it was hypothesized that any beneficial dietary modulation of the intestinal environment should reflect in composition andactivitiesofthececalmicroflora.Therefore,biomarkersrelatingtoparameters ofcecalmicrobialecologysuch as microflora composition, concentration of volatile fatty acids, and bacterial glycolytic enzyme activities were also determined.