The samples were added
with 2 ll of gel loading buffer (0.25% (w/v) bromophenol blue, 40%
(w/v) sucrose) and then loaded onto wells with 1% (w/v) agarose
gel made up in TAE buffer (40 mM Tris–acetate, 1 mM EDTA (ethylenediaminetetraacetic
acid), pH 8.3) pre-strained with 0.002%
(w/v) ethidium bromide (EtBr). This gel was run at a constant voltage
(50 V cm1) for approximately 1 h.