Aggregation and surface hydrophobicity are two important indicators used to evaluate the successful
development of biogranules. The effect of substrate concentration, pH, and temperature on these
properties of selected bacteria that are isolated from biogranules used in treating textile wastewater was
investigated. Statistical response surface methodology was used to quantitatively determine the effects
and to determine the relationship between the responses and variables. Thirty experimental runs were
performed at substrate concentrations of 500e3000 mg L1, pH of 5e9, and temperatures of 20e40 C.
The results show that all variables investigated were either positively or negatively correlated with
aggregation and surface hydrophobicity and induced significant linear responses in these indicators.
Interaction effects were also observed for some of the variables. An acceptable statistical model
describing the relationship between aggregation and surface hydrophobicity of the selected bacteria and
substrate concentration, pH, and temperature has been developed for the stated experimental
conditions.
Aggregation and surface hydrophobicity are two important indicators used to evaluate the successful
development of biogranules. The effect of substrate concentration, pH, and temperature on these
properties of selected bacteria that are isolated from biogranules used in treating textile wastewater was
investigated. Statistical response surface methodology was used to quantitatively determine the effects
and to determine the relationship between the responses and variables. Thirty experimental runs were
performed at substrate concentrations of 500e3000 mg L1, pH of 5e9, and temperatures of 20e40 C.
The results show that all variables investigated were either positively or negatively correlated with
aggregation and surface hydrophobicity and induced significant linear responses in these indicators.
Interaction effects were also observed for some of the variables. An acceptable statistical model
describing the relationship between aggregation and surface hydrophobicity of the selected bacteria and
substrate concentration, pH, and temperature has been developed for the stated experimental
conditions.
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