Ashriyani (2009) who studied the production of E. cottonii biethanol using cellulose enzymes produced from T.
viride mentioned that the hydrolysis process to get a reduction sugar only reached 48 hours and declined after that.
This can be caused by two things. Firstly, glucose inhibits the cellulose enzyme thus reducing the enzyme's activity.
Second reason was T. viride is to use the glucose from the hydrolysis as a carbon source that impact the glucose
production will also be reduced. The results of this study are also similar to that occurred in the study of Ashriyani
(2009). The cellulose enzyme used in this study will use glucose as a carbon source. Thus, after the 12 hours the
cellulose enzyme and substrate were not balanced anymore.