nuclear Citrus leprosis virus (CiLV-N) from Queretaro state of Mexico and Citrus tristeza virus (genotypes T30, T36 and T68), Citrus ring spot virus,Citrus tatter leaf virus, Citrus leaf rugose virus from the green house of Citrus Research and Education Center, Lake Alfred were assayed to determine the specificity of developed primers and probe of CiLV-C.