2.4 Culture Initiation
The damaged ends of the sterile explants were
spliced off with a sterile scalpel into 2 cm long pieces.
They were then inoculated on the culture media and
the culture bottles labeled with the variety type and
date of culture. The cultures were then transferred into
the growth room where they were arranged in a
completely randomized design with nine replications
per variety and incubated at a temperature of 28 °C
with a photoperiod of 16 hours light and eight hours
darkness. The cultures were regularly checked and the
progress in leaf formation, node development, root
production and plant height recorded at intervals of 14
days for six weeks.