It has been reported that hydrophobic
bonds in protein structures are weakened at low temperature.
Sawamura, Nagaoka, and Machikawa (2001) reported that the hydrophobic
groups of proteins are stabilized by hydration at low temperature.
Hirano and Shiraki (2011) also reported that cold denaturation of proteins occurs due to hydrophobic hydration. Our previous work reported
that water-insoluble pesticide residues result from hydrophobic
interactions, and that an increasing removal rate can be interpreted as
weakening of these interactions at high pressure and low temperature,
which is identical to the general accepted theory in this field