A long term evolutionary adapted yeast strain was obtained
after 65 days’ adaptation (equivalent to 780 generations) by successively
transferring in the corn stover hydrolysate containing
high inhibitor substances. The adapted yeast strain demonstrated
significantly improved ethanol fermentation performance during
SSF at high solids content. The ethanol titer and yield of 71.40 g/L
and 80.34% were obtained, respectively, at an optimum SSF condition
without any wastewater generation from pretreatment to fermentation.
The present study provides a practical approach for
improving fermentability of microorganisms used in lignocellulose
biorefinery.