Fig. 5. (A) Agar double diffusion test showing appearance of precipitation lines due
to biosurfactant produced by B. methylotrophicus USTBa. (a) First well was filled
with 20 mM sodium dodecyl sulfate (SDS) and the second with 50 mM barium
chloride (b). Both the wells were filled with biosurfactant containing culture
supernantant of B. methylotrophicus USTBa. (B) Emulsification activity (E24) of the
biosurfactant against different hydrocarbons and oils.