3.3. Seed cryopreservation by vitrification
Results showed that viability and germination rates of freshly
harvested seeds of B. formosana were the highest. The viability rates
of seeds not treated with sucrose solution and PVS2 for 1 h and
3 h were 58.1% and 62.4%, respectively (Table 2). The rates were
lower than 50% using pre-culture with 0.06 M, with 0.3 M sucrose
solution. The viability and germination rates of seeds pre-cultured
in sucrose solution and PVS2 were lower than that of fresh seeds
and those desiccated by silica gel or air-drying (Tables 1 and 2).