CONCLUSION
A simple isocratic HPLC-DAD
method was successfully developed for the
determination of benzoic acid and sorbic acid
in noodles. The sample preparation engaged
the extraction of sample with methanol: water
(60:40, v/v) that was economic and easy to
accomplish without using harmful organic
solvent or time-consuming and expensive
solid-phase extraction as previous report
[19,22,24]. The complete separation of the
analytes were achieved in shorter
chromatographic run time (3 min) in
comparison to all published literature [19-24]
and found to be more specific for noodle
sample. All validation parameters were within
the acceptance range. This proposed method
was applied for the analysis of benzoic acid
and sorbic acid in the different kinds of
noodles and shown satisfying results in terms
of accuracy, repeatability and easiness.
Additionally, reducing run time caused less in
using reagents. The advantage of utilizing
fewer reagents could also bring the
healthier life and cleaner environment.