Automated DNA sequencing electropherograms were proofed
and edited using CodonCode Aligner v. 1.6.3. Sequences were truncated
to include only the ITS2 region. The DNA sequences from Gen-
Bank were annotated and trimmed using ITS2 annotation tools
based on Hidden Markov Model (HMM) (Keller et al., 2009). Sequences
of plant samples were aligned with those from GenBank
by ClustalX software and the genetic distances were calculated
using Kimura 2-Parameter (K2P) model. The distributions of intraversus
inter-specific variability were compared using DNA barcoding
gap with the software TAXON DNA (Slabbinck et al., 2008). Wilcoxon
signed-rank tests were performed as previously described
(Yao et al., 2010) (Table 1). Moreover, BLAST 1 and the nearest distance
method were used to test the power of species identification
as described previously (Yao et al., 2010).