Abstract goby, Oxyeleotris marmoratus is a carnivorous fish that highly demanded in Asia region and has great potential in aquaculture industry. A feeding trial was conducted to investigate the possibility of eplacing fishmeal with soybean meal in the diet for marble goby juvenile. Fish (initial body weight and length 2.60+0.04 cm respectively) were fed with four isonitrogenous and isolipidic diets, which contained 0%, 10% and 20% of defatted soybean meal (SB0, SB10 and SB20, respectively) and 20% of soybean supplemented with 2000 FTUnkg fish were randomly distributed into 12L and hand-fed apparent satiation twice daily. After 40-day of feeding trial, highest growth was observed in fish fed SB0, without significant difference with fish SB10; both groups were significant better than juveniles fed SB20 and SB20+P Treatment SB20+P obtained slightly higher growth than those fe SB20. Similar trend was observed in the specific growth rate, feed conversion ratio and nitrogen retention efficiency. Protein efficiency ratio of fish fed SB0, SB10 and SB20+P was significant higher than fish fed The body lipid content was significantly reduced in higher soybean et due to starvation as the fish were reluctant to feed on experimental No symptoms was observed in juveniles fed d SB10 while juveniles fed SB20+P had better intestinal morphology than in SB20. conclusion, young marble goby juvenile can utilize 10% of defatted soybean meal in In their diet without affecting its growth, nutrient utilization and intestinal condition