The concentration of ethanol is estimated using NUCON 5765 gas chromatography(GC) with a flame ionization detector(FID) and CHROMATOPAK(10% Carbowax 20M) column(3m length and 1/8 mm diameter) using N, as the carrier gas at the rate of 30 per minute. Hydrogen and compressed air are used as fuel gas and are regulated at a rate of 30 and 300 Hl per minute respectively Flame is ignited at the flame ionization detector port detector injector and oven temperatures are programmed. After reaching the stability, when the oven temperature and detector, injector emperature are at the programmed temperature, a sample s injected from fermentation flask into injector port by using a micro syringe(1 10 ul). The oven temperature is held at 80°C. The injector and detector temperature is maintained at 200°C. Injection volume is 1HL and 0.760 g/ml absolute ethanol is used as the internal standard. The peak eluted is noted(using WINACDS 6.2 software) and by knowing the area of peak, the concentration of ethanol calculated using calibration chart. A view of the Gas chromatography used for this study is shown in Fig. 4.2 and the ethanol analysis